The midfielder was hooked at halftime after a nightmare first 45 minutes in Nigeria's 2-1 defeat to England at Wembley on Saturday, and told KweséESPN that it was a "disappointing game" for him.
The substitutes are Daniel Akpeyi, Ikechukwu Ezenwa, Kenneth Omeruo, Mikel Agu, Chidozie Awaziem, Simeon Nwankwo, Kelechi Iheanacho, Ahmed Musa, John Ogu, Tyronne Ebuehi, Ola Aina, Elderson Echiejile, Oghenekaro Etebo and Wilfred Ndidi.
England face Costa Rica at Elland Road in Leeds on Thursday before jetting off to Russian Federation to let the real preparations commence.
But the England manager will also have to consider having another talk with Sterling after the City winger's latest controversy.
Southgate will no doubt be pleased with the win, but concerned at a sloppy start to the second-half by his charges.
The 23-year-old spurned a number of chances to score at Wembley before being cautioned for diving in the second half.
Nigeria had their first glimpse of the ball in the final third when, as the clock ran down, Victor Moses and Alex Iwobi became a bit more lively on the wings but they failed to connect with the attack.More news: NBA Finals 2018: Warriors Win Game 1 Despite LeBron's Heroics
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It was that kind of evening for Southgate, with pluses and negatives in equal measure.
Arsenal's Alex Iwobi scored straight after the restart to give the 10,000 Nigeria fans inside the national stadium something to shout about.
"We don't think it was the moment for him to play but he will start the next game but I hope if he plays against Czech Republic, he will be fit and ready on June 16". They survived an early free-kick whipped in by Kieran Tripper, with goalkeeper Francis Uzoho dealing with the floated delivery.
Alli, who has a Nigerian father, didn't seem perturbed and he was soon celebrating when England took a seventh-minute lead.
Everton goalkeeper Jordan Pickford, making only his third global appearance, hardly had a save to make in the first half.
The Super Eagles needed a second-half response and quickly delivered after a change of shape to match their opponents, Iwobi side-footing in after Ighalo's shot came back off the post.